Utility of Peptide Nucleic Acid Fluorescence In Situ Hybridization for Rapid Detection of Acinetobacter spp. and Pseudomonas aeruginosa | Zendy

Anton Y. Peleg | Zendy; Yaphet Tilahun | Zendy; Mark J. Fiandaca | Zendy; Erika M. C. D’Agata | Zendy; Lata Venkataraman | Zendy; Robert C. Moellering | Zendy; George M. Eliopoulos | Zendy

Open Access

Utility of Peptide Nucleic Acid Fluorescence In Situ Hybridization for Rapid Detection of Acinetobacter spp. and Pseudomonas aeruginosa

Author(s) -

Anton Y. Peleg,

Yaphet Tilahun,

Mark J. Fiandaca,

Erika M. C. D’Agata,

Lata Venkataraman,

Robert C. Moellering,

George M. Eliopoulos

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01724-08

Subject(s) - peptide nucleic acid , acinetobacter , pseudomonas aeruginosa , microbiology and biotechnology , nucleic acid , biology , bacteria , pseudomonas , pseudomonadaceae , pseudomonadales , nucleic acid thermodynamics , fluorescence in situ hybridization , hybridization probe , dna , biochemistry , antibiotics , gene , base sequence , genetics , chromosome

The utility of peptide nucleic acid fluorescence in situ hybridization (PNA FISH) for the detection of Acinetobacter spp. and Pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of ATCC strains and clinical isolates with select gram-negative rods. After testing 60 clinical isolates, PNA FISH had a sensitivity and specificity of 100% and 100%, respectively, for Acinetobacter spp. and 100% and 95%, respectively, for P. aeruginosa. PNA FISH was able to detect both pathogens simultaneously and directly from spiked blood cultures.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research