Interlaboratory Comparison of Intact-Cell Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Results for Identification and Differentiation of Brucella spp | Zendy

Axel Karger | Zendy; Falk Melzer | Zendy; Markus Timke | Zendy; Barbara Bettin | Zendy; Markus Kostrzewa | Zendy; Karsten Nöckler | Zendy; Angelika Hohmann | Zendy; Herbert Tomaso | Zendy; Heinrich Neubauer | Zendy; Sascha Al Dahouk | Zendy

Open Access

Interlaboratory Comparison of Intact-Cell Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Results for Identification and Differentiation of Brucella spp

Author(s) -

Axel Karger,

Falk Melzer,

Markus Timke,

Barbara Bettin,

Markus Kostrzewa,

Karsten Nöckler,

Angelika Hohmann,

Herbert Tomaso,

Heinrich Neubauer,

Sascha Al Dahouk

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01720-13

Subject(s) - mass spectrometry , brucella , identification (biology) , chromatography , chemistry , biology , microbiology and biotechnology , brucellosis , virology , botany

Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification ofBrucella spp. Analysis of mass peak patterns allowed accurate identification to the genus level. However, statistical models based on peak intensities were needed for definite species differentiation. Interlaboratory comparison confirmed the reproducibility of the results.

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