Open AccessExternal Quality Assessment of SARS-CoV-2 Sequencing: an ESGMD-SSM Pilot Trial across 15 European Laboratories
Author(s) -
Fanny Wegner,
Tim Roloff,
Michael Huber,
Samuel Cordey,
Alban Ramette,
Yannick Gerth,
Claire Bertelli,
Madlen Stange,
Helena M. B. Seth-Smith,
Alfredo Mari,
Karoline Leuzinger,
Lorenzo Cerutti,
Keith Harshman,
Ioannis Xénarios,
Philippe Le Mercier,
Pascal Bittel,
Stefan Neuenschwander,
Onya Opota,
Jonas Fuchs,
Marcus Panning,
Charlotte Michel,
Marie Hallin,
Thomas Demuyser,
Ricardo De Mendonça,
Paul H. M. Savelkoul,
Jozef Dingemans,
Brian van der Veer,
Stefan A. Boers,
Eric C. J. Claas,
Jordy P. M. Coolen,
Willem J. G. Melchers,
Marianne Gunell,
Teemu Kallonen,
Tytti Vuorinen,
Antti Hakanen,
Eva Bernhoff,
Marit Andrea Klokkhammer Hetland,
Hadar Golan Berman,
Sheera Adar,
Jacob Moran-Gilad,
Dana Wolf,
Stephen L. Leib,
Oliver Nolte,
Laurent Kaiser,
Stefan Schmutz,
Verena Kufner,
Maryam Zaheri,
Alexandra Trkola,
Hege Vangstein Aamot,
Hans H. Hirsch,
Gilbert Greub,
Adrian Egli
Publication year - 2022
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01698-21
Subject(s) - external quality assessment , clinical microbiology , covid-19 , dna sequencing , molecular diagnostics , whole genome sequencing , genomic sequencing , sars virus , virology , computational biology , medicine , medical physics , biology , genome , infectious disease (medical specialty) , bioinformatics , microbiology and biotechnology , genetics , pathology , outbreak , gene , disease
This first pilot trial on external quality assessment (EQA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) whole-genome sequencing, initiated by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group for Genomic and Molecular Diagnostics (ESGMD) and the Swiss Society for Microbiology (SSM), aims to build a framework between laboratories in order to improve pathogen surveillance sequencing. Ten samples with various viral loads were sent out to 15 clinical laboratories that had free choice of sequencing methods and bioinformatic analyses.