Open AccessCohort Study of Molecular Identification and Typing of Mycobacterium abscessus, Mycobacterium massiliense , and Mycobacterium bolletii
Author(s) -
Adrian M. Zelazny,
Jeremy M. Root,
Yvonne R. Shea,
Rhonda E Colombo,
Isdore Chola Shamputa,
Frida Stock,
Sean Conlan,
Steven G. McNulty,
Barbara A. BrownElliott,
Richard J. Wallace,
Kenneth N. Olivier,
Steven M. Holland,
Elizabeth P. Sampaio
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01688-08
Subject(s) - microbiology and biotechnology , mycobacterium , nontuberculous mycobacteria , typing , mycobacterium abscessus , biology , mycobacterium infections , virology , identification (biology) , bacteria , genetics , botany
Mycobacterium abscessus is the most common cause of rapidly growing mycobacterial chronic lung disease. Recently, two newM. abscessus -related species,M. massiliense andM. bolletii , have been described. Health care-associated outbreaks have recently been investigated by the use of molecular identification and typing tools; however, very little is known about the natural epidemiology and pathogenicity ofM. massiliense orM. bolletii outside of outbreak situations. The differentiation of these two species fromM. abscessus is difficult and relies on the sequencing of one or more housekeeping genes. We performed extensive molecular identification and typing of 42 clinical isolates ofM. abscessus, M. massiliense , andM. bolletii from patients monitored at the NIH between 1999 and 2007. The corresponding clinical data were also examined. Partial sequencing ofrpoB, hsp65 , andsecA led to the unambiguous identification of 26M. abscessus isolates, 7M. massiliense isolates, and 2M. bolletii isolates. The identification results for seven other isolates were ambiguous and warranted further sequencing and an integrated phylogenetic analysis. Strain relatedness was assessed by repetitive-sequence-based PCR (rep-PCR) and pulsed-field gel electrophoresis (PFGE), which showed the characteristic clonal groups for each species. Five isolates with ambiguous species identities asM. abscessus -M. massiliense byrpoB, hsp 65 , andsecA sequencing clustered as a distinct group by rep-PCR and PFGE together with theM. massiliense type strain. Overall, the clinical manifestations of disease caused by each species were similar. In summary, a multilocus sequencing approach (not justrpoB partial sequencing) is required for division ofM. abscessus and closely related species. Molecular typing complements sequence-based identification and provides information on prevalent clones with possible relevant clinical aspects.