Evaluation of a Multitarget Real-Time PCR Assay for Detection of Bordetella Species during a Pertussis Outbreak in New Hampshire in 2011 | Zendy

Fengxiang Gao | Zendy; Jennifer C. Mahoney | Zendy; Elizabeth R. Daly | Zendy; Wendy Lamothe | Zendy; Daniel Tullo | Zendy; Christine Bean | Zendy

Open Access

Evaluation of a Multitarget Real-Time PCR Assay for Detection of Bordetella Species during a Pertussis Outbreak in New Hampshire in 2011

Author(s) -

Fengxiang Gao,

Jennifer C. Mahoney,

Elizabeth R. Daly,

Wendy Lamothe,

Daniel Tullo,

Christine Bean

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01656-13

Subject(s) - bordetella pertussis , outbreak , pertussis toxin , microbiology and biotechnology , whooping cough , polymerase chain reaction , biology , bordetella , virology , real time polymerase chain reaction , bacteria , g protein , genetics , gene , vaccination , receptor

A multitarget real-time PCR assay with three targets, including insertion sequence481 (IS481 ), IS1001 , and an IS1001 -like element, as well as pertussis toxin subunit S1 (ptxS1 ), for the detection ofBordetella species was evaluated during a pertussis outbreak. The sensitivity and specificity were 77 and 88% (PCR) and 66 and 100% (culture), respectively. All patients with an IS481 CT of <30 also tested positive byptxS1 assay and were clinical pertussis cases. No patients with IS481 CT values of ≥40 tested positive by culture. Therefore, we recommend that culture be performed only for specimens with IS481 CT values of 30 ≤CT <40.

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