Open AccessMolecular Phylogeny and Proposal of Two New Species of the Emerging Pathogenic FungusSaksenaea
Author(s) -
Enrique José Varela Álvarez,
Dea GarciaHermoso,
Deanna A. Sutton,
J. Cano,
Alberto M. Stchigel,
Damien Hoinard,
Annette W. Fothergill,
Michael G. Rinaldi,
Françoise Dromer,
Josep Guarro
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01646-10
Subject(s) - phylogenetics , fungus , molecular phylogenetics , biology , pathogenic fungus , evolutionary biology , computational biology , zoology , genetics , botany , gene
Saksenaea is a monotypic genus belonging to the orderMucorales and capable of producing severe human infections. Through a polyphasic study based on analysis of the sequences of the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene, and the elongation factor 1α (EF-1α) gene, as well as by evaluation of relevant morphological and physiological characteristics of a set of clinical and environmental strains, we have demonstrated thatSaksenaea vasiformis is a complex of species. We propose as new speciesSaksenaea oblongispora , characterized by oblong sporangiospores and unable to grow at 42°C, andSaksenaea erythrospora , characterized by large sporangiophores and sporangia and by ellipsoid sporangiospores, biconcave in the lateral view. Itraconazole, posaconazole, and terbinafine were active against all isolates included in the study, while amphotericin B, voriconazole, and the echinocandins showed low activity.