Screening for Human Immunodeficiency Virus Infection by Use of a Fourth-Generation Antigen/Antibody Assay and Dried Blood Spots: In-Depth Analysis of Sensitivity and Performance Assessment in a Cross-Sectional Study | Zendy

Karl Stéfic | Zendy; Jérôme Guinard | Zendy; Gilles Peytavin | Zendy; Leïla Saboni | Zendy; Cécile Sommen | Zendy; Claire Sauvage | Zendy; Florence Lot | Zendy; Syria Laperche | Zendy; Annie Velter | Zendy; Françis Barin | Zendy

Open Access

Screening for Human Immunodeficiency Virus Infection by Use of a Fourth-Generation Antigen/Antibody Assay and Dried Blood Spots: In-Depth Analysis of Sensitivity and Performance Assessment in a Cross-Sectional Study

Author(s) -

Karl Stéfic,

Jérôme Guinard,

Gilles Peytavin,

Leïla Saboni,

Cécile Sommen,

Claire Sauvage,

Florence Lot,

Syria Laperche,

Annie Velter,

Françis Barin

Publication year - 2019

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01645-19

Subject(s) - antibody , dried blood , dried blood spot , population , medicine , immunoassay , human immunodeficiency virus (hiv) , antigen , immunology , cross sectional study , fourth generation , virology , biology , third generation , pathology , chemistry , environmental health , chromatography , genetics , telecommunications , computer science

We evaluated the performance of a fourth-generation antigen/antibody (Ag/Ab) assay for detecting HIV-1 infection on dried blood spots (DBS) both in a conventional laboratory environment and in an epidemiological survey corresponding to a real-life situation. Although a 2-log loss of sensitivity compared to that with plasma was observed when using DBS in an analytical analysis, the median delay of positivity between DBS and crude serum during the early phase postacute infection was 7 days.

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