Open AccessCharacterization of Slackia exigua Isolated from Human Wound Infections, Including Abscesses of Intestinal Origin
Author(s) -
Keun-Sung Kim,
Michael Rowlinson,
Robert S. Bennion,
Chengxu Liu,
David A. Talan,
Paula Summanen,
Sydney M. Finegold
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01576-09
Subject(s) - exigua , biology , fastidious organism , microbiology and biotechnology , 16s ribosomal rna , ribosomal rna , sequence analysis , gene , bacteria , genetics , spodoptera , recombinant dna
Eleven clinical strains isolated from infected wound specimens were subjected to polyphasic taxonomic analysis. Sequence analysis of the 16S rRNA gene showed that all 11 strains were phylogenetically related toSlackia exigua . Additionally, conventional and biochemical tests of 6 of the 11 strains were performed as supplementary methods to obtain phenotypic identification by comparison with the phenotypes of the relevant type strains.S. exigua has been considered an oral bacterial species in the familyCoriobacteriaceae . This organism is fastidious and grows poorly, so it may easily be overlooked. The 16S rRNA gene sequences and the biochemical characteristics of four of theS. exigua strains isolated for this study from various infections indicative of an intestinal source were almost identical to those of the validatedS. exigua type strain from an oral source and two of theS. exigua strains from oral sources evaluated in this study. Thus, we show for the first time thatS. exigua species can be isolated from extraoral infections as well as from oral infections. The profiles of susceptibility to selected antimicrobials of this species were also investigated for the first time.