Evaluation of Escherichia coli Cell Response to Antibiotic Treatment by Use of Raman Spectroscopy with Laser Tweezers | Zendy

Tobias J. Moritz | Zendy; Christopher R. Polage | Zendy; Douglas S. Taylor | Zendy; Denise M. Krol | Zendy; Stephen M. Lane | Zendy; James W. Chan | Zendy

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Evaluation of Escherichia coli Cell Response to Antibiotic Treatment by Use of Raman Spectroscopy with Laser Tweezers

Author(s) -

Tobias J. Moritz,

Christopher R. Polage,

Douglas S. Taylor,

Denise M. Krol,

Stephen M. Lane,

James W. Chan

Publication year - 2010

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01565-10

Subject(s) - escherichia coli , raman spectroscopy , microbiology and biotechnology , antibiotics , streptomycin , cefazolin , tweezers , penicillin , optical tweezers , enterobacteriaceae , biology , chemistry , optics , biochemistry , physics , gene

Laser tweezers Raman spectroscopy was used to detect the cellular response of Escherichia coli cells to penicillin G-streptomycin and cefazolin. Time-dependent intensity changes of several Raman peaks at 729, 1,245, and 1,660 cm(-1) enabled untreated cells and cells treated with the different antibiotic drugs to be distinguished.

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