Open AccessIdentification of Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization
Author(s) -
Sven Poppert,
Michaela Haas,
Tatjana Yildiz,
Thomas Alter,
Edda Bartel,
Ursula Fricke,
Andreas Essig
Publication year - 2008
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01512-07
Subject(s) - campylobacter , biology , arcobacter , microbiology and biotechnology , campylobacter coli , campylobacter jejuni , fluorescence in situ hybridization , helicobacter , bacteria , genetics , helicobacter pylori , gene , chromosome
ThermotolerantCampylobacter spp. (Campylobacter jejuni ,C. coli ,C. lari , andC. upsaliensis ) are leading causes of food-borne diarrhea in humans. In this study, the usefulness of fluorescence in situ hybridization (FISH) for the identification ofCampylobacter isolates was investigated. A hierarchical FISH probe set that included six group-, genus-, and species-specific probes was developed and evaluated with 12 reference strains and 94 clinical isolates ofCampylobacter ,Arcobacter , andHelicobacter . FISH correctly identified all isolates to the genus level and detected all thermotolerantCampylobacter isolates. The assay showed high degrees of sensitivity for the identification ofC. jejuni (90%),C. coli (97%),C. lari (81%), andC. upsaliensis (100%) to the species level.