Identification of Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization | Zendy

Sven Poppert | Zendy; Michaela Haas | Zendy; Tatjana Yildiz | Zendy; Thomas Alter | Zendy; Edda Bartel | Zendy; Ursula Fricke | Zendy; Andreas Essig | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Identification of Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization

Author(s) -

Sven Poppert,

Michaela Haas,

Tatjana Yildiz,

Thomas Alter,

Edda Bartel,

Ursula Fricke,

Andreas Essig

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01512-07

Subject(s) - campylobacter , biology , arcobacter , microbiology and biotechnology , campylobacter coli , campylobacter jejuni , fluorescence in situ hybridization , helicobacter , bacteria , genetics , helicobacter pylori , gene , chromosome

ThermotolerantCampylobacter spp. (Campylobacter jejuni ,C. coli ,C. lari , andC. upsaliensis ) are leading causes of food-borne diarrhea in humans. In this study, the usefulness of fluorescence in situ hybridization (FISH) for the identification ofCampylobacter isolates was investigated. A hierarchical FISH probe set that included six group-, genus-, and species-specific probes was developed and evaluated with 12 reference strains and 94 clinical isolates ofCampylobacter ,Arcobacter , andHelicobacter . FISH correctly identified all isolates to the genus level and detected all thermotolerantCampylobacter isolates. The assay showed high degrees of sensitivity for the identification ofC. jejuni (90%),C. coli (97%),C. lari (81%), andC. upsaliensis (100%) to the species level.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research