Open AccessMultiCode-RTx Real-Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples
Author(s) -
Evguenia S. Svarovskaia,
Michael J. Moser,
Andrew Bae,
James R. Prudent,
Michael D. Miller,
Katyna Borroto–Esoda
Publication year - 2006
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01512-06
Subject(s) - reverse transcriptase , virology , biology , virus , mutant , population , human immunodeficiency virus (hiv) , polymerase chain reaction , gene , genetics , medicine , environmental health
We report a real-time PCR assay capable of detecting drug-resistant human immunodeficiency virus type 1 reverse transcriptase K65R mutant virus at a level of 0.5% in polymorphic patient plasma specimens. Fifty-three treatment-naïve and 20 treatment-experienced specimens were successfully genotyped with the new method. Results were in agreement with population sequencing and the labor-intensive single-genome sequencing method.