MultiCode-RTx Real-Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples | Zendy

Evguenia S. Svarovskaia | Zendy; Michael J. Moser | Zendy; Andrew Bae | Zendy; James R. Prudent | Zendy; Michael D. Miller | Zendy; Katyna Borroto–Esoda | Zendy

Open Access

MultiCode-RTx Real-Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples

Author(s) -

Evguenia S. Svarovskaia,

Michael J. Moser,

Andrew Bae,

James R. Prudent,

Michael D. Miller,

Katyna Borroto–Esoda

Publication year - 2006

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01512-06

Subject(s) - reverse transcriptase , virology , biology , virus , mutant , population , human immunodeficiency virus (hiv) , polymerase chain reaction , gene , genetics , medicine , environmental health

We report a real-time PCR assay capable of detecting drug-resistant human immunodeficiency virus type 1 reverse transcriptase K65R mutant virus at a level of 0.5% in polymorphic patient plasma specimens. Fifty-three treatment-naïve and 20 treatment-experienced specimens were successfully genotyped with the new method. Results were in agreement with population sequencing and the labor-intensive single-genome sequencing method.

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