Newly Developed Primers for Comprehensive Amplification of the rpoB Gene and Detection of Rifampin Resistance in Mycobacterium tuberculosis | Zendy

Leen Rigouts | Zendy; Oscar Nolasco | Zendy; Pim de Rijk | Zendy; Elie Nduwamahoro | Zendy; Armand Van Deun | Zendy; Andy Ramsay | Zendy; Jorge Arévalo | Zendy; Françoise Portaels | Zendy

Open Access

Newly Developed Primers for Comprehensive Amplification of the rpoB Gene and Detection of Rifampin Resistance in Mycobacterium tuberculosis

Author(s) -

Leen Rigouts,

Oscar Nolasco,

Pim de Rijk,

Elie Nduwamahoro,

Armand Van Deun,

Andy Ramsay,

Jorge Arévalo,

Françoise Portaels

Publication year - 2006

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01489-06

Subject(s) - rpob , mycobacterium tuberculosis , tuberculosis , biology , polymerase chain reaction , virology , microbiology and biotechnology , gene , genetics , medicine , pathology

New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.

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