Open AccessNewly Developed Primers for Comprehensive Amplification of the rpoB Gene and Detection of Rifampin Resistance in Mycobacterium tuberculosis
Author(s) -
Leen Rigouts,
Oscar Nolasco,
Pim de Rijk,
Elie Nduwamahoro,
Armand Van Deun,
Andy Ramsay,
Jorge Arévalo,
Françoise Portaels
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01489-06
Subject(s) - rpob , mycobacterium tuberculosis , tuberculosis , biology , polymerase chain reaction , virology , microbiology and biotechnology , gene , genetics , medicine , pathology
New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.