Open AccessDetection and Differentiation of Two Koala Gammaherpesviruses by Use of High-Resolution Melt (HRM) Analysis Reveals Differences in Viral Prevalence and Clinical Associations in a Large Study of Free-Ranging Koalas
Author(s) -
Paola K. Vaz,
Alistair R. Legione,
Carol A. Hartley,
Joanne M. Devlin
Publication year - 2019
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01478-18
Subject(s) - phascolarctos cinereus , biology , high resolution melt , virology , host (biology) , high resolution , polymerase chain reaction , genetics , population , gene , medicine , remote sensing , environmental health , geology
The iconic koala (Phascolarctos cinereus ) is host to two divergent gammaherpesviruses, phascolarctid gammaherpesviruses 1 and 2 (PhaHV-1 and -2), but the clinical significance of the individual viruses is unknown and current diagnostic methods are unsuitable for differentiating between the viruses in large-scale studies. To address this, we modified a pan-herpesvirus nested PCR to incorporate high-resolution melt analysis.
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