Open AccessDual-Reporter Phenotypic Assay for Human Immunodeficiency Viruses
Author(s) -
Keiko Kajiwara,
Eiichi Kodama,
Yasuko Sakagami,
Toshio Naito,
Masao Matsuoka
Publication year - 2008
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01470-07
Subject(s) - reporter gene , virology , biology , human immunodeficiency virus (hiv) , phenotype , long terminal repeat , viral replication , virus , gene , microbiology and biotechnology , genetics , gene expression
We have established a novel human immunodeficiency virus (HIV) tandem-reporter assay using HIV receptor-transduced NP-2 cells with long terminal repeat-controlled β-galactosidase, inserted internal ribosome entry site, and secretary alkaline phosphatase genes. This assay allows users to detect replication of clinical isolates, indicating its useful application as an HIV phenotypic assay.