Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry as an Alternative to 16S rRNA Gene Sequencing for Identification of Difficult-To-Identify Bacterial Strains | Zendy

Alain Bizzini | Zendy; Katia Jaton | Zendy; Dolly Revelo Romo | Zendy; Jacques Billé | Zendy; Guy Prod’hom | Zendy; Gilbert Greub | Zendy

Open Access

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry as an Alternative to 16S rRNA Gene Sequencing for Identification of Difficult-To-Identify Bacterial Strains

Author(s) -

Alain Bizzini,

Katia Jaton,

Dolly Revelo Romo,

Jacques Billé,

Guy Prod’hom,

Gilbert Greub

Publication year - 2010

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01463-10

Subject(s) - 16s ribosomal rna , mass spectrometry , biology , computational biology , ribosomal rna , matrix assisted laser desorption/ionization , identification (biology) , gene , bacterial taxonomy , dna sequencing , microbiology and biotechnology , chromatography , genetics , desorption , chemistry , botany , organic chemistry , adsorption

Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16S rRNA gene sequencing. MALDI-TOF MS might represent an alternative to 16S rRNA gene sequencing.

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