Detection of Mumps Virus RNA by Real-Time One-Step Reverse Transcriptase PCR Using the LightCycler Platform | Zendy

Jason J. LeBlanc | Zendy; Janice Pettipas | Zendy; Ross Davidson | Zendy; Graham Tipples | Zendy; Joanne Hiebert | Zendy; Todd F. Hatchette | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Detection of Mumps Virus RNA by Real-Time One-Step Reverse Transcriptase PCR Using the LightCycler Platform

Author(s) -

Jason J. LeBlanc,

Janice Pettipas,

Ross Davidson,

Graham Tipples,

Joanne Hiebert,

Todd F. Hatchette

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01446-08

Subject(s) - reverse transcriptase , mumps virus , virology , real time polymerase chain reaction , biology , reverse transcription polymerase chain reaction , rna , rna directed dna polymerase , virus , messenger rna , genetics , gene

A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research