Comparison of BD GeneOhm Cdiff and Seegene Seeplex ACE PCR Assays Using Toxigenic Clostridium difficile Culture for Direct Detection of tcdB from Stool Specimens | Zendy

Bo-Moon Shin | Zendy; Se Jin Mun | Zendy; Soo Jin Yoo | Zendy; Eun Young Kuak | Zendy

Open Access

Comparison of BD GeneOhm Cdiff and Seegene Seeplex ACE PCR Assays Using Toxigenic Clostridium difficile Culture for Direct Detection of tcdB from Stool Specimens

Author(s) -

Bo-Moon Shin,

Se Jin Mun,

Soo Jin Yoo,

Eun Young Kuak

Publication year - 2012

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01440-12

Subject(s) - concordance , clostridium difficile , microbiology and biotechnology , predictive value , medicine , biology , antibiotics

We evaluated the performances of 2 PCR assays (BD GeneOhm and Seegene ACE) for direct detection of tcdB from stool specimens. The concordance rate between BD and Seegene was 96.3%. The sensitivities, specificities, positive predictive values (PPVs), and negative predictive values (NPVs) of BD and Seegene were 95.7%, 96.5%, 91.8%, and 98.2% and 90.0%, 97.1%, 92.6%, and 96.0%, respectively.

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