Open AccessMultiplex PCR-Ligation Detection Reaction Assay for Simultaneous Detection of Drug Resistance and Toxin Genes from Staphylococcus aureus , Enterococcus faecalis , and Enterococcus faecium
Author(s) -
Kathleen Granger,
Mark S. Rundell,
Maneesh Pingle,
Rebecca Shatsky,
Davise H. Larone,
Linnie M. Golightly,
Francis Barany,
Eric D. Spitzer
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01411-09
Subject(s) - enterococcus faecium , microbiology and biotechnology , enterococcus faecalis , staphylococcus aureus , multiplex polymerase chain reaction , biology , multiplex , enterococcus , drug resistance , toxin , micrococcaceae , antibacterial agent , polymerase chain reaction , virology , bacteria , antibiotics , gene , genetics
A multiplex PCR-ligation detection reaction (PCR-LDR) assay was developed for rapid detection of methicillin, tetracycline, and vancomycin resistance, as well as toxic shock toxin and Panton-Valentine leukocidin. The assay was tested on 470 positive blood culture bottles containingStaphylococcus aureus or enterococci. PCR-LDR exhibited a sensitivity and specificity of ≥98% for all components except tetracycline resistance, which had a sensitivity of 94.7%. Rapid and sensitive detection of antimicrobial resistance and virulence genes could help guide therapy and appropriate infection control measures.