Colony Multiplex-Tandem PCR for Rapid, Accurate Identification of Fungal Cultures | Zendy

Anna F. Lau | Zendy; Tania C. Sorrell | Zendy; OkCha Lee | Zendy; Keith K. Stanley | Zendy; Catriona Halliday | Zendy

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Colony Multiplex-Tandem PCR for Rapid, Accurate Identification of Fungal Cultures

Author(s) -

Anna F. Lau,

Tania C. Sorrell,

OkCha Lee,

Keith K. Stanley,

Catriona Halliday

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01411-08

Subject(s) - multiplex polymerase chain reaction , biology , multiplex , isolation (microbiology) , polymerase chain reaction , microbiology and biotechnology , identification (biology) , variants of pcr , genetics , gene , botany

We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.

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