Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates | Zendy

Elena Castellanos | Zendy; Alicia Aranaz | Zendy; Beatriz Romero | Zendy; Lucía de Juan | Zendy; Julio Álvarez | Zendy; Javier Bezos | Zendy; Sabrina Rodríguez | Zendy; Karen Stevenson | Zendy; Ana Mateos | Zendy; Lucas Domı́nguez | Zendy

Open Access

Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates

Author(s) -

Elena Castellanos,

Alicia Aranaz,

Beatriz Romero,

Lucía de Juan,

Julio Álvarez,

Javier Bezos,

Sabrina Rodríguez,

Karen Stevenson,

Ana Mateos,

Lucas Domı́nguez

Publication year - 2007

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01411-07

Subject(s) - biology , paratuberculosis , restriction fragment length polymorphism , mycobacterium , microbiology and biotechnology , genotype , gene , restriction enzyme , genetics , bacteria

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

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