Detection and Toxin Typing ofClostridium perfringensin Formalin-Fixed, Paraffin-Embedded Tissue Samples by PCR | Zendy

Josephine W. Wu | Zendy; Wandi Zhang | Zendy; Boxun Xie | Zendy; Maoxin Wu | Zendy; Xiaodi Tong | Zendy; Jayant Kalpoe | Zendy; David Zhang | Zendy

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Detection and Toxin Typing ofClostridium perfringensin Formalin-Fixed, Paraffin-Embedded Tissue Samples by PCR

Author(s) -

Josephine W. Wu,

Wandi Zhang,

Boxun Xie,

Maoxin Wu,

Xiaodi Tong,

Jayant Kalpoe,

David Zhang

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01324-08

Subject(s) - clostridium perfringens , microbiology and biotechnology , biology , typing , toxin , clostridiaceae , polymerase chain reaction , gene , bacteria , genetics

Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.

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