PCR Identification of Bacteria in Blood Culture Does Not Fit the Daily Workflow of a Routine Microbiology Laboratory | Zendy

Santra Karumaa | Zendy; Pauliina Kärpänoja | Zendy; Hannu Sarkkinen | Zendy

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PCR Identification of Bacteria in Blood Culture Does Not Fit the Daily Workflow of a Routine Microbiology Laboratory

Author(s) -

Santra Karumaa,

Pauliina Kärpänoja,

Hannu Sarkkinen

Publication year - 2011

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01271-11

Subject(s) - blood culture , microbiology and biotechnology , genotype , bacteria , biology , identification (biology) , clinical microbiology , antibiotics , genetics , ecology , gene

We have evaluated the GenoType blood culture assay (Hain Lifescience, Nehren, Germany) for the identification of bacteria in 233 positive blood cultures and assessed its suitability in the workflow of a routine microbiology laboratory. In 68/233 (29.2%) samples, the culture result could not be confirmed by the GenoType assay due to a lack of primers in the test, multiple organisms in the sample, or inconsistency with respect to the identification by culture. Although the GenoType blood culture assay gives satisfactory results for bacteria for which primers are available, there are difficulties in applying the test in the routine microbiology laboratory.

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