Open AccessModeling Uncertainty in Single-Copy Assays for HIV
Author(s) -
Rutao Luo,
Michael J. Piovoso,
Ryan Zurakowski
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01254-12
Subject(s) - confidence interval , human immunodeficiency virus (hiv) , sensitivity (control systems) , biology , virology , computational biology , genetics , statistics , mathematics , electronic engineering , engineering
We present a simple computational model of measurement accuracy for single-copy sensitivity assays (SCA) of HIV RNA that was developed from first principles. The model shows that the SCA is significantly right-skewed. Measured virus concentrations of 1 and 10 virions/ml had overlapping 95% confidence intervals and were statistically indistinguishable.
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