Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus ( Sensu Stricto ) | Zendy

Shih-Hua Teng | Zendy; Chang−Mu Chen | Zendy; MengRui Lee | Zendy; Tai-Fen Lee | Zendy; KunYi Chien | Zendy; LeeJene Teng | Zendy; PoRen Hsueh | Zendy

Open Access

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus ( Sensu Stricto )

Author(s) -

Shih-Hua Teng,

Chang−Mu Chen,

MengRui Lee,

Tai-Fen Lee,

KunYi Chien,

LeeJene Teng,

PoRen Hsueh

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01239-13

Subject(s) - sensu stricto , mycobacterium abscessus , mass spectrometry , subspecies , matrix assisted laser desorption/ionization , microbiology and biotechnology , mycobacterium , biology , chemistry , desorption , chromatography , bacteria , genetics , zoology , organic chemistry , adsorption

Among 36Mycobacterium masilliense and 22M. abscessus isolates identified byerm (41) PCR and sequencing analysis ofrpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization–time of flight mass spectrometry.

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