Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples | Zendy

Tracy D. Lee | Zendy; K. Adie | Zendy; A. McNabb | Zendy; Dale Purych | Zendy; Kulvinder Mannan | Zendy; Robert Azana | Zendy; Corrinne Ng | Zendy; Patrick Tang | Zendy; Linda Hoang | Zendy

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Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples

Author(s) -

Tracy D. Lee,

K. Adie,

A. McNabb,

Dale Purych,

Kulvinder Mannan,

Robert Azana,

Corrinne Ng,

Patrick Tang,

Linda Hoang

Publication year - 2015

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01237-15

Subject(s) - microbiology and biotechnology , real time polymerase chain reaction , medicine , virology , biology , genetics , gene

We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-β-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichment broth lysates.

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