Open AccessImpact of Long-Term Storage on Stability of Standard DNA for Nucleic Acid-Based Methods
Author(s) -
Barbara Röder,
Karin Frühwirth,
Claus Vogl,
Martin Wagner,
Peter Rossmanith
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01230-10
Subject(s) - nucleic acid , dna , degradation (telecommunications) , term (time) , distilled water , glycerol , chemistry , biology , biological system , chromatography , biochemistry , computer science , physics , telecommunications , quantum mechanics
Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a deviation of 0.2 threshold cycle (C(T)) values resulted after 100 days of storage.
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