Open AccessDetection of Oseltamivir Resistance during Treatment of 2009 H1N1 Influenza Virus Infection in Immunocompromised Patients: Utility of Cycle Threshold Values of Qualitative Real-Time Reverse Transcriptase PCR
Author(s) -
Tejal Mehta,
Eric McGrath,
Suchitha Bheemreddy,
Hossein Salimnia,
Nahed AbdelHaq,
Jocelyn Y. Ang,
Lawrence G. Lum,
Pranatharthi H. Chandrasekar,
George Alangaden
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01190-10
Subject(s) - oseltamivir , virology , reverse transcriptase , viral shedding , drug resistance , virus , biology , viral load , reverse transcription polymerase chain reaction , pneumonia , pyrosequencing , influenza a virus , real time polymerase chain reaction , polymerase chain reaction , immunology , covid-19 , medicine , microbiology and biotechnology , gene , infectious disease (medical specialty) , disease , biochemistry , messenger rna
Two immunocompromised patients with 2009 H1N1 influenza pneumonia had viral shedding for over 5 weeks despite therapy with oseltamivir. Declining or persistently low cycle threshold values noted on serial qualitative real-time reverse transcriptase PCR (rRT-PCR) of respiratory specimens implied increasing viral load and probable drug resistance. Oseltamivir resistance was later confirmed by pyrosequencing.