Open AccessVancomycin MIC for Methicillin-Resistant Coagulase-Negative Staphylococcus Isolates: Evaluation of the Broth Microdilution and Etest Methods
Author(s) -
Rodrigo Minuto Paiva,
Alice Beatriz Mombach Pinheiro Machado,
Alexandre Prehn Zavascki,
Afonso Luís Barth
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01182-10
Subject(s) - etest , broth microdilution , microbiology and biotechnology , vancomycin , coagulase , methicillin resistant staphylococcus aureus , micrococcaceae , staphylococcus , staphylococcus aureus , medicine , biology , antibacterial agent , minimum inhibitory concentration , antibiotics , bacteria , genetics
Vancomycin MIC results were determined by the broth microdilution (BMD) method and by Etest using 130 methicillin-resistant coagulase-negative staphylococcus bloodstream isolates obtained from a tertiary hospital. The majority (98.5%) of MIC results determined by BMD were ≤1 μg/ml, in contrast to MIC results determined by Etest (72.3% were ≥1.5 μg/ml). The MICs obtained by Etest were, in general, 1- to 2-fold higher than the MICs obtained by BMD.