Open AccessRelatedness of Human and Animal Clostridium difficile PCR Ribotype 078 Isolates Determined on the Basis of Multilocus Variable-Number Tandem-Repeat Analysis and Tetracycline Resistance
Author(s) -
Dennis Bakker,
Jeroen Corver,
Céline Harmanus,
Abraham Goorhuis,
E. C. Keessen,
Warren N. Fawley,
Mark H. Wilcox,
Ed J. Kuijper
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01171-10
Subject(s) - multiple loci vntr analysis , tetracycline , biology , clostridium difficile , microbiology and biotechnology , multilocus sequence typing , tandem repeat , genetics , variable number tandem repeat , genotype , gene , antibiotics , genome
Totals of 102 and 56 Clostridium difficile type 078 strains of human and porcine origins, respectively, from four European countries were investigated by an optimized multilocus variable-number tandem-repeat analysis (MLVA) and for tetracycline susceptibility. Eighty-five percent of all isolates were genetically related, irrespective of human or porcine origin. Human strains were significantly more resistant to tetracycline than porcine strains. All tetracycline-resistant strains contained the Tn916-like transposon harboring the tet(M) gene. We conclude that strains from human and porcine origins are genetically related, irrespective of the country of origin. This may reflect a lack of diversity and/or common source.
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