Rapid Simultaneous Detection by Real-Time PCR of Cytomegalovirus UL97 Mutations in Codons 460 and 520 Conferring Ganciclovir Resistance | Zendy

Katharina Göhring | Zendy; Elfriede Mikeler | Zendy; Gerhard Jahn | Zendy; Klaus Hamprecht | Zendy

Open Access

Rapid Simultaneous Detection by Real-Time PCR of Cytomegalovirus UL97 Mutations in Codons 460 and 520 Conferring Ganciclovir Resistance

Author(s) -

Katharina Göhring,

Elfriede Mikeler,

Gerhard Jahn,

Klaus Hamprecht

Publication year - 2006

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01141-06

Subject(s) - ganciclovir , human cytomegalovirus , virology , biology , cytomegalovirus , restriction fragment length polymorphism , betaherpesvirinae , polymerase chain reaction , gene , herpesviridae , virus , genetics , viral disease

Ganciclovir (GCV) resistance is an emerging problem for transplant recipients. A sensitive and rapid real-time PCR approach for simultaneous and semiquantitative detection of human cytomegalovirus (HCMV) UL97 mutations in codons 460/520 was established by LightCycler and confirmed by restriction fragment length polymorphism and sequencing. Results from HCMV laboratory strains were compared with results from 11 GCV-resistant clinical isolates.

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