Open AccessUse of Peptide Nucleic Acid-Fluorescence In Situ Hybridization for Definitive, Rapid Identification of Five Common Candida Species
Author(s) -
Megan E. Reller,
Amanda B. Mallonee,
Nicole P. Kwiatkowski,
William G. Merz
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01127-07
Subject(s) - peptide nucleic acid , nucleic acid , fluorescence in situ hybridization , biology , fish <actinopterygii> , microbiology and biotechnology , in situ , peptide , fluorescence , in situ hybridization , biochemistry , chemistry , gene , messenger rna , physics , organic chemistry , quantum mechanics , fishery , chromosome
We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with fiveCandida species-specific probes to identifyCandida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify thoseCandida species isolated most frequently.