Open AccessAmplified Fragment Length Polymorphism Reveals Specific Epigenetic Distinctions between Mycobacterium avium Subspecies paratuberculosis Isolates of Various Isolation Types
Author(s) -
B. O'Shea,
Sangeeta Khare,
Patricia E. Klein,
Allen J. Roussel,
L. Garry Adams,
Thomas A. Ficht,
Allison C. RiceFicht
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01123-10
Subject(s) - amplified fragment length polymorphism , biology , paratuberculosis , restriction enzyme , genetics , sequence analysis , restriction fragment length polymorphism , genotype , mycobacterium avium subspecies paratuberculosis , feces , microbiology and biotechnology , genetic variation , mycobacterium , genetic analysis , genetic diversity , dna , gene , bacteria , population , demography , sociology
Amplified fragment length polymorphism (AFLP) was employed as a genetic analysis tool for the study of the genetic relatedness ofMycobacterium avium subsp.paratuberculosis isolates harvested from bovine fecal samples and from bovine or human tissues. This analysis revealed genetic differences between these two isolate types that were confirmed through cluster analysis. Dendrogram analysis separated these two isolate types based on the isolation scheme (tissue-associated versus fecalM. avium subsp.paratuberculosis isolates). Further sequence analysis of unique genetic regions from each isolation type revealed no genetic sequence differences. However, Clustal DNA alignments identified AFLP restriction enzyme sites that were undigested in the tissue-associated isolates. AFLP analysis also disclosed that the same AFLP restriction sites were digested in all of the fecal isolates. Sequence analysis further revealed a consensus sequence upstream of the undigested restriction sites for possible methyltransferase recognition in the tissue-associatedM. avium subsp.paratuberculosis isolates.