Open AccessIntimin Types Determined by Heteroduplex Mobility Assay of Intimin Gene ( eae )-Positive Escherichia coli Strains
Author(s) -
Kenitiro Ito,
Mariko Iida,
Mitsugu Yamazaki,
Kazuo Moriya,
Sanae Moroishi,
Jun Yatsuyanagi,
Takayuki Kurazono,
Noriaki Hiruta,
OrnAg Ratchtrachenchai
Publication year - 2007
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01103-06
Subject(s) - intimin , heteroduplex , escherichia coli , biology , gene , microbiology and biotechnology , virology , genetics , enterobacteriaceae
We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes.
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