Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay | Zendy

Viola Maass | Zendy; Jan Marco Kern | Zendy; Matthias Poeckl | Zendy; Matthias Maass | Zendy

Open Access

Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay

Author(s) -

Viola Maass,

Jan Marco Kern,

Matthias Poeckl,

Matthias Maass

Publication year - 2011

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01092-11

Subject(s) - mycoplasma , biology , chlamydiaceae , chlamydia , mollicutes , microbiology and biotechnology , chlamydiales , polymerase chain reaction , mycoplasmataceae , contamination , virology , mycoplasma pneumoniae , chlamydophila pneumoniae , primer (cosmetics) , genetics , gene , pneumonia , medicine , chemistry , ecology , organic chemistry

Mycoplasma contamination is a frequent problem in chlamydial cell culture. After obtaining contradictory contamination results, we compared three commercial PCR kits for mycoplasma detection. One kit signaled contamination in mycoplasma-free Chlamydia pneumoniae cultures. Sequencing of cloned PCR products revealed primer homology with the chlamydial genome as the basis of this false-positive result.

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