Open AccessHigh-Resolution Melting Analysis for Identification of the Cryptococcus neoformans-Cryptococcus gattii Complex
Author(s) -
Sara Gago,
Óscar Zaragoza,
Isabel Cuesta,
Juan Luis Rodríguez-Tudela,
Manuel CuencaEstrella,
María José Buitrago
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01091-11
Subject(s) - cryptococcus neoformans , cryptococcus gattii , cryptococcosis , cryptococcus , biology , high resolution melt , microbiology and biotechnology , genetics , polymerase chain reaction , gene
We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from theCryptococcus species complex (Cryptococcus neoformans var.grubii ,Cryptococcus neoformans var.neoformans , andCryptococcus gattii ). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the mainCryptococcus species.