Formic Acid-Based Direct, On-Plate Testing of Yeast and Corynebacterium Species by Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry | Zendy

Elitza S. Theel | Zendy; Bryan H. Schmitt | Zendy; Leslie Hall | Zendy; Scott A. Cunningham | Zendy; Robert C. Walchak | Zendy; Robin Patel | Zendy; Nancy L. Wengenack | Zendy

Open Access

Formic Acid-Based Direct, On-Plate Testing of Yeast and Corynebacterium Species by Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Author(s) -

Elitza S. Theel,

Bryan H. Schmitt,

Leslie Hall,

Scott A. Cunningham,

Robert C. Walchak,

Robin Patel,

Nancy L. Wengenack

Publication year - 2012

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01045-12

Subject(s) - formic acid , mass spectrometry , yeast , chromatography , matrix assisted laser desorption/ionization , matrix (chemical analysis) , desorption , chemistry , microbiology and biotechnology , biology , biochemistry , organic chemistry , adsorption

An on-plate testing method using formic acid was evaluated on the Bruker Biotyper matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry system using 90 yeast and 78Corynebacterium species isolates, and 95.6 and 81.1% of yeast and 96.1 and 92.3% ofCorynebacterium isolates were correctly identified to the genus and species levels, respectively. The on-plate method using formic acid yielded identification percentages similar to those for the conventional but more laborious tube-based extraction.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research