Controlled Comparison of BacT/Alert MB System, Manual Myco/F Lytic Procedure, and Isolator 10 System for Diagnosis of Mycobacterium tuberculosis Bacteremia | Zendy

John A. Crump | Zendy; Anne B. Morrissey | Zendy; Habib O. Ramadhani | Zendy; Boniface Njau | Zendy; Venance P. Maro | Zendy; L. Barth Reller | Zendy

Open Access

Controlled Comparison of BacT/Alert MB System, Manual Myco/F Lytic Procedure, and Isolator 10 System for Diagnosis of Mycobacterium tuberculosis Bacteremia

Author(s) -

John A. Crump,

Anne B. Morrissey,

Habib O. Ramadhani,

Boniface Njau,

Venance P. Maro,

L. Barth Reller

Publication year - 2011

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.01035-11

Subject(s) - lytic cycle , bacteremia , mycobacterium tuberculosis , isolator , tuberculosis , microbiology and biotechnology , mycobacterium , virology , medicine , isolation (microbiology) , biology , pathology , antibiotics , virus , electronic engineering , engineering

We compared the performance of the BacT/Alert MB system, that of the manual Bactec Myco/F Lytic procedure, and that of the Isolator 10 lysis-centrifugation system in the detection of Mycobacterium tuberculosis bacteremia. Mean times to detection were 16.4 days for BacT/Alert MB versus 20.0 days for Myco/F Lytic, 16.5 days for BacT/Alert MB versus 23.8 days for Isolator 10, and 21.1 days for Bactec Myco/F Lytic versus 22.7 days for Isolator 10. There were no significant differences in yields. The mean (range) magnitude of mycobacteremia was 30.0 (0.4, 90.0) CFU/ml and was correlated with the time to positivity in the BacT/Alert MB system (r = -0.4920). M. tuberculosis bacteremia was detected more rapidly in a continuously monitored liquid blood culture system, but the mean time to positivity exceeded 3 weeks.

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