Open AccessPerformance of the Aptima HIV-1 RNA Qualitative Assay with 16- and 32-Member Specimen Pools
Author(s) -
Steven F. Ethridge,
Clyde E. Hart,
Debra L. Hanson,
Monica M. Parker,
Timothy J. Sullivan,
Berry Bennett,
Petrice Stephens,
Joslyn Hilliard,
Pragna Patel
Publication year - 2010
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01030-10
Subject(s) - rna , virology , human immunodeficiency virus (hiv) , biology , lentivirus , genetics , viral disease , gene
The Aptima HIV-1 RNA qualitative assay tested with a WHO-approved HIV type 1 RNA standard in 16- and 32-member pools detected 100% of the pools (1,070 and 2,130 HIV-1 RNA copies/ml/pool, respectively), thus exceeding the FDA-required lower limit of detection. The Aptima test can be used to screen for acute-phase HIV infection.