Open AccessNovel Sensitive Real-Time PCR for Quantification of Bacterial 16S rRNA Genes in Plasma of HIV-Infected Patients as a Marker for Microbial Translocation
Author(s) -
Marit Kramski,
Adriana Gaeguta,
Gregor F. Lichtfuss,
Reena Rajasuriar,
Suzanne Crowe,
Martyn A. French,
Sharon R. Lewin,
Damian Purcell
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.01018-11
Subject(s) - 16s ribosomal rna , biology , nuclease , gene , chromosomal translocation , real time polymerase chain reaction , polymerase chain reaction , microbiology and biotechnology , ribosomal rna , virology , genetics
We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.