Open AccessFalse-Negative Results Obtained with the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test Caused by Unrecognized Inhibition of the Amplification Reaction
Author(s) -
Ji Yeon Kim,
Mary Jane Ferraro,
John A. Branda
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00966-09
Subject(s) - mycobacterium tuberculosis , mycobacterium , microbiology and biotechnology , false negative reactions , tuberculosis , gene duplication , biology , virology , genetics , bacteria , medicine , pathology , gene , radiology
The Gen-Probe AmplifiedMycobacterium tuberculosis direct test is widely used in the diagnosis of tuberculosis. Specimens may contain amplification inhibitors, potentially leading to false-negative results if unrecognized. We report a failure to detect inhibition, despite adherence to the inhibition testing guidelines provided on the label, and recommend changes.