Open AccessGenetic Lineages of Community-Associated Methicillin-Resistant Staphylococcus aureus in Kuwait Hospitals
Author(s) -
Edet E. Udo,
Frances O’Brien,
Noura AlSweih,
Bobby Noronha,
Bassignani Matthew,
W.B. Grubb
Publication year - 2008
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00966-08
Subject(s) - pulsed field gel electrophoresis , multilocus sequence typing , microbiology and biotechnology , biology , staphylococcus aureus , typing , allotype , virology , genetics , gene , genotype , bacteria
Twenty-six community-associated methicillin-resistantStaphylococcus aureus (CAMSRA) isolates were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and screened for accessory gene regulator (agr), capsular polysaccharide (cap), and Panton-Valentine leucocidin (PVL) genes. They exhibited five PFGE patterns (types A to E). The majority were PFGE type A (12 isolates) or type B (8 isolates). MLST showed that PFGE type A isolates belonged to sequence type 80 (ST80), while the PFGE type B isolates were ST30. The ST80 and ST30 clones contained agr allotype 3, cap type 8, and PVL. The results showed that two internationally recognized CAMRSA clones are dominant in Kuwait hospitals.