Use of Reverse Transcription-PCR-Based Assays for Quantification of HIV-1 in Dried Blood Spots Requires Specific HIV-1 RNA Isolation for Monitoring of Antiretroviral Treatment Efficiency | Zendy

François Rouet | Zendy; Dramane Kania | Zendy; Édouard Tuaillon | Zendy; Almoustapha Issiaka Maïga | Zendy; Janin Nouhin | Zendy; Christine Rouzioux | Zendy; Diane Descamps | Zendy; JeanChristophe Plantier | Zendy

Open Access

Use of Reverse Transcription-PCR-Based Assays for Quantification of HIV-1 in Dried Blood Spots Requires Specific HIV-1 RNA Isolation for Monitoring of Antiretroviral Treatment Efficiency

Author(s) -

François Rouet,

Dramane Kania,

Édouard Tuaillon,

Almoustapha Issiaka Maïga,

Janin Nouhin,

Christine Rouzioux,

Diane Descamps,

JeanChristophe Plantier

Publication year - 2014

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00962-14

Subject(s) - dried blood spot , reverse transcriptase , virology , dried blood , viral load , human immunodeficiency virus (hiv) , rna , biology , drug resistance , isolation (microbiology) , spots , antiretroviral treatment , rna extraction , lentivirus , viral disease , antiretroviral therapy , microbiology and biotechnology , genetics , gene , chemistry , chromatography , botany

The paper published by the ANRS 12235 Study Group ([1][1]) showed that monitoring of “real-life” HIV-1 RNA viral load (VL) and drug resistance mutations (DRMs) in dried blood spot (DBS) specimens collected from patients in Africa and Asia was possible, but with some limitations, particularly for

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