Open AccessFalse-Positive PCR Detection of Tropheryma whipplei in Cerebrospinal Fluid and Biopsy Samples from a Child with Chronic Lymphocytic Meningitis
Author(s) -
Daniel Goyo,
Ana Camacho,
Carmen Gómez,
Rogelio Simón de las Heras,
Joaquín R. Otero,
Fernando Cháves
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00927-09
Subject(s) - tropheryma whipplei , cerebrospinal fluid , biology , whipple disease , meningitis , pathology , biopsy , ribosomal rna , whipple's disease , microbiology and biotechnology , polymerase chain reaction , medicine , gene , disease , genetics , coeliac disease , psychiatry , intestinal malabsorption
We report the case of a teenager with chronic lymphocytic meningitis for whomTropheryma whipplei 16S rRNA PCR results were positive in two cerebrospinal fluid samples and one duodenal biopsy specimen. PCR targeting another specific sequence ofTropheryma whipplei and sequencing of the initially amplified 16S rRNA fragment did not confirm the results.