Open AccessRapid Direct Susceptibility Testing from Positive Blood Cultures by the Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry-Based Direct-on-Target Microdroplet Growth Assay
Author(s) -
Evgeny A. Idelevich,
Luise M. Storck,
Katrin Sparbier,
Oliver Drews,
Markus Kostrzewa,
Karsten Becker
Publication year - 2018
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00913-18
Subject(s) - mass spectrometry , desorption , matrix (chemical analysis) , chromatography , matrix assisted laser desorption/ionization , ionization , laser , chemistry , time of flight mass spectrometry , analytical chemistry (journal) , materials science , physics , optics , ion , organic chemistry , adsorption
The recently developed direct-on-target microdroplet growth assay (DOT-MGA) allows rapid universal antimicrobial susceptibility testing (AST) using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). Here, we investigated a direct application of this method on positive blood cultures (BCs) for the acceleration of sepsis diagnostics.
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