Open AccessRecovery of Anti- Toxoplasma gondii Immunoglobulin M in Stored Guthrie Card Blood Spots
Author(s) -
H Van Tan,
Eskild Petersen,
Line Møller,
Pamela Phillips,
Eurico Camargo Neto,
Ruth Gilbert
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00893-09
Subject(s) - toxoplasmosis , toxoplasma gondii , antibody , immunoglobulin m , dried blood , filter paper , spots , immunology , immunoglobulin g , virology , medicine , biology , chromatography , chemistry , pathology
Retrospective testing of neonatal Guthrie card blood spots for specific immunoglobulin M (IgM) can distinguish congenital toxoplasmosis from acquired toxoplasmosis. We determined whether storage temperature reduced IgM detection, using filter paper blood samples “spiked” with anti-Toxoplasma IgM. After 300 days, IgM detection deteriorated with storage at room temperature but not at temperatures of 4°C or lower.