Evaluation of Cycloserine-Cefoxitin Fructose Agar (CCFA), CCFA with Horse Blood and Taurocholate, and Cycloserine-Cefoxitin Mannitol Broth with Taurocholate and Lysozyme for Recovery of Clostridium difficile Isolates from Fecal Samples | Zendy

Kerin L. Tyrrell | Zendy; Diane M. Citron | Zendy; Eliza S. Leoncio | Zendy; C. Vreni Merriam | Zendy; Ellie J. C. Goldstein | Zendy

Open Access

Evaluation of Cycloserine-Cefoxitin Fructose Agar (CCFA), CCFA with Horse Blood and Taurocholate, and Cycloserine-Cefoxitin Mannitol Broth with Taurocholate and Lysozyme for Recovery of Clostridium difficile Isolates from Fecal Samples

Author(s) -

Kerin L. Tyrrell,

Diane M. Citron,

Eliza S. Leoncio,

C. Vreni Merriam,

Ellie J. C. Goldstein

Publication year - 2013

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00879-13

Subject(s) - cefoxitin , cycloserine , clostridium difficile , mannitol , microbiology and biotechnology , lysozyme , agar , biology , feces , fructose , antibiotics , food science , biochemistry , bacteria , genetics , staphylococcus aureus

Cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate (CCFA-HT), and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) were compared for recovery of Clostridium difficile from 120 stool specimens. Compared to CCFA, CCFA-HT enhanced C. difficile growth and improved recovery by 4%. In a separate study, 9% (8/91) of stool samples previously C. difficile negative on plate medium were C. difficile positive when cultured in CCMB-TAL.

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