Open AccessInterleukin-28B Genotyping by Melt-Mismatch Amplification Mutation Assay PCR Analysis Using Single Nucleotide Polymorphisms rs12979860 and rs8099917, a Useful Tool for Prediction of Therapy Response in Hepatitis C Patients
Author(s) -
Salvador Fonseca-Coronado,
Gilberto Vaughan,
Mayra Cruz-Rivera,
Juan Carlos Carpio-Pedroza,
Karina Ruíz-Tovar,
Juan Alberto Ruíz-Pacheco,
Alejandro EscobarGutiérrez
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00877-11
Subject(s) - genotyping , single nucleotide polymorphism , interleukin 28b , hepatitis c virus , biology , virology , genotype , mutation , hepatitis c , genetics , virus , gene
Several studies have identified associations between single nucleotide polymorphisms (SNPs) occurring near the interleukin-28B (IL-28B) gene and response to antiviral treatment among hepatitis C virus (HCV) patients. Here, we describe a reliable melt-mismatch amplification mutation assay (melt-MAMA) PCR-based genotyping method for IL-28B which can be used in the management of HCV patients, helping to better define the course of therapy.