Open AccessIdentification of Coagulase-Negative Staphylococci by Using the BD Phoenix System in the Low-Inoculum Mode
Author(s) -
Gioconda Brigante,
Maria Grazia Menozzi,
Beatrice Pini,
Rosaria Porta,
P Somenzi,
A Sciacca,
Teresa Spanu,
Stefania Stefani
Publication year - 2008
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00877-08
Subject(s) - coagulase , microbiology and biotechnology , staphylococcus saprophyticus , staphylococcus haemolyticus , staphylococcus epidermidis , micrococcaceae , biology , staphylococcus , 16s ribosomal rna , gene , bacteria , staphylococcus aureus , genetics , antibacterial agent , antibiotics
The new “low-inoculum” mode of the Phoenix system was evaluated to identify clinical coagulase-negative staphylococci. API ID32 Staph panels were used as comparators, and discrepancies were resolved by 16S rRNA andtuf gene analysis. The system correctly identified 90.5% of isolates, with a mean time of 10.2 h. Accuracy was satisfactory forStaphylococcus epidermidis, S. saprophyticus , andS. haemolyticus .