Detection of Group B Streptococci in Lim Broth by Use of Group B Streptococcus Peptide Nucleic Acid Fluorescent In Situ Hybridization and Selective and Nonselective Agars | Zendy

Naomi Montague | Zendy; Timothy J. Cleary | Zendy; Octavio Martínez | Zendy; Gary W. Procop | Zendy

Open Access

Detection of Group B Streptococci in Lim Broth by Use of Group B Streptococcus Peptide Nucleic Acid Fluorescent In Situ Hybridization and Selective and Nonselective Agars

Author(s) -

Naomi Montague,

Timothy J. Cleary,

Octavio Martínez,

Gary W. Procop

Publication year - 2008

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00858-08

Subject(s) - peptide nucleic acid , streptococcus , agar , group b , group a , biology , fluorescence in situ hybridization , microbiology and biotechnology , nucleic acid , agar plate , streptococcus agalactiae , streptococcaceae , fish <actinopterygii> , fluorescence , bacteria , biochemistry , gene , genetics , antibiotics , medicine , physics , quantum mechanics , fishery , chromosome

The sensitivity, specificity, and positive and negative predictive values for the detection of group B streptococci from Lim enrichment broth with sheep blood agar (SBA), with selectiveStreptococcus agar (SSA), and by a peptide nucleic acid fluorescent in situ hybridization (PNA FISH) assay were as follows: for culture on SBA, 68.4%, 100%, 100%, and 87.9%, respectively; for culture on SSA, 85.5%, 100%, 100%, and 94.1%, respectively; and for the PNA FISH assay, 97.4%, 98.3%, 96.1%, and 98.9%, respectively.

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