Open AccessDetection of Group B Streptococci in Lim Broth by Use of Group B Streptococcus Peptide Nucleic Acid Fluorescent In Situ Hybridization and Selective and Nonselective Agars
Author(s) -
Naomi Montague,
Timothy J. Cleary,
Octavio Martínez,
Gary W. Procop
Publication year - 2008
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00858-08
Subject(s) - peptide nucleic acid , streptococcus , agar , group b , group a , biology , fluorescence in situ hybridization , microbiology and biotechnology , nucleic acid , agar plate , streptococcus agalactiae , streptococcaceae , fish <actinopterygii> , fluorescence , bacteria , biochemistry , gene , genetics , antibiotics , medicine , physics , quantum mechanics , fishery , chromosome
The sensitivity, specificity, and positive and negative predictive values for the detection of group B streptococci from Lim enrichment broth with sheep blood agar (SBA), with selectiveStreptococcus agar (SSA), and by a peptide nucleic acid fluorescent in situ hybridization (PNA FISH) assay were as follows: for culture on SBA, 68.4%, 100%, 100%, and 87.9%, respectively; for culture on SSA, 85.5%, 100%, 100%, and 94.1%, respectively; and for the PNA FISH assay, 97.4%, 98.3%, 96.1%, and 98.9%, respectively.