Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens | Zendy

Nuno Guimarães | Zendy; Nuno F. Azevedo | Zendy; Céu Figueiredo | Zendy; C. W. Keevil | Zendy; M. J. Vieira | Zendy

Open Access

Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens

Author(s) -

Nuno Guimarães,

Nuno F. Azevedo,

Céu Figueiredo,

C. W. Keevil,

M. J. Vieira

Publication year - 2007

Publication title -

journal of clinical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.349

H-Index - 255

eISSN - 1070-633X

pISSN - 0095-1137

DOI - 10.1128/jcm.00858-07

Subject(s) - peptide nucleic acid , helicobacter pylori , nucleic acid , biology , fish <actinopterygii> , pathogen , fluorescence in situ hybridization , peptide , spirillaceae , biopsy , nucleic acid thermodynamics , gastric biopsy , microbiology and biotechnology , helicobacter , biochemistry , pathology , gene , gastritis , medicine , rna , genetics , fishery , chromosome

In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens.

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